The Mercedes-Benz Museum aims to enable all visitors to enjoy its offerings without any restrictions, ensuring equal treatment and the right of self-determination for everyone, regardless of any disabilities. The realisation of these aims brought the museum the 2007 architecture award for "exemplary barrier-free design".

Barrier-free access to and movement within the exhibition was accorded top priority well before the opening. Ramps around the Mercedes-Benz Museum facilitate access for wheelchair users. The "Legend" exhibition rooms also incorporate DIN-standard ramps with intermediate landings and handrails at wheelchair-compatible height. The materials used at the museum have also been chosen with the aim of ensuring a barrier-free environment, as demonstrated by non-slip flooring throughout the building.

Guests with physical disabilities receive special consideration at the Mercedes-Benz Museum. They are able to use a group ticket counter, in order to avoid strenuous waiting times. On request, the museum also offers special guided tours for wheelchair users. Guided tours for blind visitors and people with impaired vision are also available at the Mercedes-Benz Museum. Models and exhibits which can be discovered by touching bring the exhibition at the Mercedes-Benz Museum to life for such visitors. Visitors with guide dogs are able to tour the exhibition with their dogs.

The museum's multi-storey car park offers wheelchair users special parking spaces for the disabled and a lift. The museum and the exhibition can be accessed from the car park without encountering any barriers. There are numerous lifts serving all levels throughout the exhibition. A barrier-free environment is also ensured by appropriate toilets, swivelling doors at the entrance to the exhibition, a wheelchair and zimmer frame hire service and the audio guide with neck loop for hearing aid users.